Recent Advances in Insect Research Involving Metabolic Rate Measurements Using Respirometry
October 12, 2021
Advances in Insect research with Qubit’s Low Range Respirometry Package (RP1LP)
For more than 25 years Qubit Systems has provided accurate, robust instruments for measuring insect respirometry in the laboratory and field. From drosophila to dung beetles, our single and multi-channel gas exchange systems have been used inventively and effectively to investigate effects of diet, environment and genome on insect physiology and behavior. Below, we celebrate some recent publications from RP1LP users.
Disparate regulation of IMD singaling drives sex differences in infection pathology in Drosophila melanogaster.Crystal, M.V. and Dionne M.S. (2021) PNAS August 10, 2021 118 (32)https://doi.org/10.1073/pnas.2026554118
Metabolic and physiological outputs were measured in Drosophila melanogaster with wild-type and mutant immune responses to determine if the sexes were differentially impacted by various sources of pathology. The sexes showed differential immune activity but similar bacteria-derived metabolic pathology. Expression of PGRP-LB, a female-specific, immune-inducible, negative regulator of the immune deficiency (IMD) pathway, enabled females to reduce immune activity in response to reductions in bacterial numbers. Females were more resistant to infection in the absence of PGRP-LB. The heightened regulation of the IMD signaling pathway by female, but not male, Drosophila melanogaster reduces the cost of immune activity at the expense of resistance to bacterial infection.
Metabolic rate, heat shock protein 70 (Hsp70) expression, antioxidant capacity, and oxidative damage in lipids were measured in males and females of the dung beetle Euoniticellus intermedius exposed to a sublethal dose of ivermectin – an anti-parasitic drug used in livestock. Metabolic rates were higher in ivermectin-treated males and females. Ivermectin-treated females increased their expression of Hsp70, whereas males increased their antioxidant capacity. Neither sex showed changes in the levels of oxidative damage to lipids. A process of hormesis is suggested, with exposure to a ivermectin inducing a protective mechanism against oxidative stress that is different in males and females. Ivermectin in dung from livestock represents a threat to dung beetles and other fauna, which may be mitigated by heat-shock proteins and antioxidants providing resistance.
Muscle function and homeostasis require cytokine inhibition of AKT activity in Drosophila. Kiedorf, K. et al. (2020) eLife 2020; 10.7554/eLife.51595
The immune system has a role in controlling metabolism. In Drosophila, a high-fat diet can trigger the immune system resulting in insulin resistance and a shorter lifespan. Kierdorf’s research investigated how the immune system and metabolism work together, revealing complex interactions. This involved stopping the activity of the dome receptor in the muscles of healthy flies, leading to increase in the activity of AKT, a protein critical in relaying insulin-type signals inside the cell. This caused hyperactivation of insulin signaling, decreased muscle function, deleterious changes in energy storage and expenditure, and a shorter life for the insects. Qubit’s RP1LP was used to monitor Drosophila metabolic rate.
Different amplitudes of temperature fluctuation induce distinct transcriptomic and matabolomic response in the dung beetle Phanaeus vindex. Sheldon, K.S. et al. (2020) J Exp Biol (2020) 223 (23) https://doi.org/10.1242/jeb.233239
The thermal sensitivity of metabolic rate and energetic reserves was investigated in dung beetles exposed to constant (20°C), low fluctuation (20±5°C), or high fluctuation (20±12°C) temperature treatments using respirometry (Qubit RP1LP) assessment of energetic reserves and HPLC-MS-based metabolomics. No significant differences in metabolic rate or energetic reserves were found, suggesting increased fluctuations were not energetically demanding. A de novo transcriptome was assembled and annotated, revealing non-overlapping transcriptomic and metabolomic responses of beetles exposed to different fluctuations, which was associated with post-translational modifications to histones. Thus, acclimation to different temperature fluctuations is distinct and related to increasing transcriptional plasticity. Histone modifications may underlie rapid acclimation to temperature variation.
The Q-Box RP1LP Low Range Respirometry Package ($10,000 US)
The Q-Box RP1LP Low Range Respiration Package is designed to measure the metabolic rate of small animals such as insects, reptiles, amphibians, other invertebrates and small mammals in an open-flow or stop-flow gas exchange system. The Q-Box RP1LP can even be used for measurements of CO2 and O2 exchange in colonies of fungi and microorganisms. When combined with Qubit’s Gas Switching Systems, measurements of metabolic rates and RQ are possible on up to 7 organisms (8 channel system including reference).
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wanted to touch base and thank you again for all your help with our Qubit Low-Range Respirometry Package. It worked great and was the centerpiece of our animal physiology lab last spring.
Dr. Amanda R. Falk, Department of Biology, Center College, Danville, KY