Z160 Multicultivator

Z160-OD Multi-Cultivator is designed for small scale cultivation of algae, bacteria or cyanobacteria. It consists of 8 vessels, each holding up to ca. 85 ml of cultivated suspension. The vessels are immersed in a temperature controlled waterbath. Each vessel is illuminated by an array of cool white LEDs that can generate incident irradiance, which is independently adjustable up to 1000 µmol(photon)/m2/s. Different than white colors of LEDs are also available.  Each vessel can be bubbled with gas. the composition of the gas may be controlled with an optional gas mixing system.  The gas is humidified and supplied to vessel through a manually adjustable valve manifold. Continuous culture is also available with the optional Turbidostat module.

The Z160 Multi-Cultivator is supplied with a light controlling unit that supports user-defined dynamic illumination protocols, such as, flashing lights or day/night cycles. Optional accessories are available such as: Cooling Unit AC-700, which serves for the water bath cooling below ambient levels.  The water pump for automatic water bath refilling (maintains constant water level in the bath). Turbidostat module allows continuous growth of the cultures according to predefined density conditions.  MC software and Control PC allow live data visualization and control of experiments in multiple units.   Each Multicultivator measures optical density (OD) at 680 and 720nm for each culture vessel.  OD readings are used for monitoring of cultures’ growth rates.  Simple OD View Software is included with the unit for downloading of the data to PC computer if the unit is purchased without the controller PC and MC software.  Two versions of the MC software are available: the Basic version provides control and visualization of data in up to two Z160-OD and the Advanced version can be used to control and visualize data from unlimited number of Multicultivators.

Optional Turbidostat Module may be supplied with Z160-OD for continuous growth of cultures.  It is equipped with a peristaltic pump and 8 valves that are controlled independentely by the MC software for addition of fresh medium to each culture.   Turbidostat module is fully software controlled therefore requires purchase of the optional Basic or Advanced MC Software.

Standard Z160-OD comes with a single color LED’s – cold white, warm white, red 635 nm or blue 470 nm.  Z160-MULTI system is also available where each cultivation slot is furnished with LED-based illumination of different color. Spectrum from 405 nm to 730 nm can be covered. Single cultivation slots are equipped with the dividers so as to eliminate different color penetration.

  • Flat, rectangular water reservoir with 8 slots for cultivation vessels.
  • Each vessel is 3 cm in diameter, 20 cm long, recommended working volume 85 ml.
  • LED  illumination adjustable in intensity (up to 0 to 1000 µmol(photon)/m2/s), timing and modulation independentely  for each culture, single and multiple color LED available
  • Light upgrade available for up to 2500 µmol(photon)/m2/s (optional)
  • Light controlling unit (included) supports user-defined protocols, such as, flashing light or day/night cycles.
  • Thermostat controlled water reservoir with a capacity of 5 liters. The reservoir is divided into 8 slots separated by the light-shielding plates.
  • The gas output is manually controlled. The sparging gas can be humidified and distributed into each vessel.
  • Optical density readings  in standard unit Z160-OD (680 and 720nm)
  • Cooling unit (optional).
  • Automatic water filling of the water bath (optional).
  • Control of gas composition with Gas Mixing System (optional)
  • Turbidostat module available for continuous cultivation (optional)
  • Replication of algal growth experiments
  • Multiple light treatments of the same algal strain
  • Multiple strain testing under same or different growth conditions
  • Phenotyping/Screening of strains
  • Measured Parameters: Optical density by two IR LEDs (735 nm, 680 nm), temperature of water bath
  • Number of culture vessels: 8
  • Culture Vessel Volume: 100 ml (maximum recommended working volume 85 ml)
  • Temperature control: ambient – 60 °C (standard), 5 °C below ambient – 60 °C (optional; with Cooling Unit AC-700)
  • Heating System: One 150W cartridge heater
  • LED Lighting: Intensity adjustable from 0 to 100 %, Maximum light intensity 1000 µmol(photon)/m2/s.  Light upgrade to 2500 available
  • Light Regime: Static or dynamic (sinus, daylight, pulse form)
  • One Display: System control and actual readings
  • Computer remote control with MC Software and PC controller (optional)
  • Control of Gas Flow: Manual
  • Control of Gas Composition : Optional with Gas Mixing System
  • Volume of Water Bath: 5 liters
  • Detector Wavelength Range: PIN photodiode with 665 nm-750 nm bandpass filters (optional)
  • Light Path (Optical Path for OD Measurement): 27 mm 
  • Bios: Upgradeable firmware
  • Communication Port: USB A-B
  • Material: Glass, stainless steel, silicon gasket, polycarbonate
  • Dimension: 71 x 21 x 33 cm
  • Weight: 13 kg
  • Electrical: 110-240 V
  • GRIS B., SFORZA E., MOROSINOTTO T., et al. (2017): Influence of light and temperature on growth and high-value molecules productivity from Cyanobacterium aponinum. Journal of Appl. Phycol. DOI 10.1007/s10811-017-1133-3
  • NIKOLAOU A., BERNARDI A., MENEGHESSO A., MOROSINOTTO T., and CHACHUAT B. (2015): A model of chlorophyll fluorescence in microalgae integrating photoproduction, photoinhibition and photoregulation. Journal of Biotechnology 194: Pages 91-99. DOI: 10.1016/j.jbiotec.2014.12.00
  • FLOWERS J. M., HAZZOURI K. M., PHAM G. M., et al. (2015): Whole-genome resequencing reveals extensive natural variation in the model green alga Chlamydomonas reinhardtii. The Plant Cell. Volume 27, Pages 2353-2369. DOI: 10.1105/tpc.15.00492
  • GRIS B., CAVEDON G., LENUCCI M., et al. (2015): Optimizing biomass and high value compound production in Cyanobacterium aponinum PCC 10605.Societa Botanica Italiana. Venezia.
  • MAKOWER A. K., SCHUURMANS J. M., GROTH D., et al. (2015): Transcriptomics aided dissection of the intracellular and the extracellular role of 1 microcystin in M. aeruginosa PCC 7806. Applied and Enviromental Microbiology. Volume 81, Pages 544–554. DOI:10.1128/AEM.02601-14
  • YU, J., LIBERTON M., CLIFTEN P. F., et al. (2015): Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO2. Scientific Reports 5,Pages 8132. DOI:10.1038/srep08132 (2015)
  • GRAMA B. S., CHADER S., KHELIFI D., AGATHOS S.N., and JEFFRYES C. (2014): Induction of canthaxanthin production in a Dactylococcus microalga isolated from the Algerian Sahara. Bioresource Technology 151: pp. 297-305. DOI: 10.1016/j.biortech.2013.10.073
  • GERIN S., MATHY G. AND FRANCK F. (2014): Modeling the dependence of respiration and photosynthesis upon light, acetate, carbon dioxide, nitrate and ammonium in Chlamydomonas reinhardtii using design of experiments and multiple regression. BMC Systems Biology 8: 96. DOI: 10.1186/s12918-014-0096-0
  • MIAZEK K., GOFFIN D., RICHEL A., et al. (2014):Growth of Chlorella in the presence of organic carbon: A photobioreactor study. Conference – Process of Technics 2014 – Prague.
  • ŠANTRŮČEK J., VRABALOVÁ M., ŠIMKOVÁ M., et al. (2014):). Stomatal and pavement cell density linked to leaf internal CO2 concentration. Annals of Botany. Pages 1-12. DOI: 10.1093/aob/mcu095
  • ZHANG B., WANG L., HASAN R., et al. (2014):). Characterization of a Native Algae Species Chlamydomonas debaryana: Strain Selection, Bioremediation Ability,and Lipid Characterization. BioResources. Volume 9, Pages 6130-6140.

Software

Available single color LED spectra for Z160-OD
Available Multicolor LED spectra for Z160-OD-MULTI
OD View software for download of OD and temperature data (standard with Z160-OD)
Photobioreactor software adapted to control of Multicultivator protocols and data visualization (optional)

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