The OX1LP Dissolved Oxygen Packages are based on the use of polarographic dissolved oxygen electrodes and may be used to measure photosynthesis and/or respiration in the aqueous phase, using suspensions of plant cells, animals, organelles (chloroplasts and mitochondria) and algae. It may also be used to monitor chemical and biochemical reactions that either produce or consume O2 in the aqueous phase. The Dissolved Oxygen Package comes complete with all of the instruments, accessories, software and data interface.
The OX1LP Dissolved Oxygen Packages are available with DO electrodes of different volumes from 100ul (flow-through) to 50 ml. The O2 electrode within the water-jacketed cuvette may be used for measurements of O2 consumption (or production) by biological samples or enzymatic reactions. Calibration is a simple 2-point process, and all the required accessories for calibration are supplied as part of the package.
The Dissolved Oxygen Package provides excellent accuracy and a resolution of 0.03% O2 (where air saturated water = 100%). Dissolved O2 values may be expressed in any desired units (mg/L; % dissolved O2; ppm etc) based on a simple calibration procedure. The water jacket allows calibrations and experiments to be carried out at the same temperature, or a simple temperature correction can be made in software for measurements made at different temperatures.
- Algal photosynthesis and respiration
- Bacterial respiration
- Respiration of small aquatic animals
- Aquatic insects and larvae respiration
- Mitochondrial respiration
- Chloroplast O2 production
- Cell suspension respiration
- Plant cells suspension photosynthesis
- Enzymatic Reactions that consume or produce O2
- Dissolved Oxygen Cuvette Electrode (1, 2.5, 4, 6, 30, 50 mL)
- A231 Cuvette Electrode Amplifier
- A255 Magnetic Stirrer
- A135 Stir Bar (2) and O-Ring (2)
- A131 KCl Filling Solution
- A136 Membrane Material and Filter Paper
- 10 ft Excelon Tubing for the Water Jacket
- C610 LabQuest Mini Data Interface
- C901 Logger Pro Software
- C404 Customized Setup Software
- Instructor’s and Student’s Manuals (include experiments)
- Syringes for addition of metabolites to cuvette; (A141) 10µL, (A143) 50µL, (A145) 100µL
- S172 Thermistor for measurements of sample temperature (not available for 1ml cuvette)
- A113 calibrated LED light source
- Q-Box rugged case for storage and transport of all components
Examples of Experiments for OX1LP packages:
- Measurement of Aquatic Photosynthetic O2 Evolution
- Effect of Inhibitors on Mitochondrial Electron Transport
- Analysis of Light-dependent Chloroplast Electron Transport
- Effects of Temperature on O2 Uptake by Aquatic Animals
- Kinetics of O2-consuming Enzymes
- Lamarre SG et al. Interrelationship Between Contractility, Protein Synthesis and Metabolism in Mantle of Juvenile Cuttlefish (Sepia officinalis). (2019) Frontiers in Physiology V10, 1-14 (doi: 10.3389/fphys.2019.01051)
- Sirikhachornkit et al. Increasing the Triacylglycerol Content in Dunaliella tertiolecta through Isolation of Starch-Deficient Mutants. J. Microbiol. Biotechnol. 26(5), 854–866 (2016)
- Croston, Tara L., et al. Functional deficiencies of subsarcolemmal mitochondria in the type 2 diabetic human heart. American Journal of Physiology-Heart and Circulatory Physiology 307.1 H54-H65 (2014)
- Sussarellu, Rossana, et al. Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas.” The Journal of Experimental Biology 216.9 1561-1569 (2013).
- Dickinson, Gary H., et al. Environmental salinity modulates the effects of elevated CO2 levels on juvenile hard-shell clams, Mercenaria mercenaria. The Journal of experimental biology 216.14 2607-2618 (2013).
- Iris Bauer and Andreas Kappler. Rates and Extent of Reduction of Fe(III) Compounds and O2 by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908 ( 2009).
- Carrie M. Mosher, Matthew A. Hummel, Timothy S. Tracy and Allan E. Rettie. Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9. Biochemistry Vol 47, Number 45, p11725–11734 (2008).
- Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance. Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).
- Bryce A. Mendelsohn and Jonathan D. Gitlin. Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo. Developmental Dynamics Vol 237, Issue 7, p1789–1798(2008).
- Johnson EA, Rosenberg J, McCarty RE. Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits. Biochimica et Biophysica Acta 1767:374-380 (2007)
- Johnson E A. Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis inChlamydamonas Reinhardtii. Photosynth Res vol 96:153-162 (2008)
- Charles W. Locuson, Peter M. Gannett and Timothy S. Tracy. Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129 (2006).
The following OX1LP packages are available depending on the volume of the DO electrode required:
- OX1LP-1 (1ml perspex)
- OX1LP-2.5 (2.5ml glass or perspex)
- OX1LP-4 (4ml perspex)
- OX1LP-6 (6ml glass)
- OX1LP-30 (30ml perspex)
- OX1LP-50 (50ml perspex or glass)
Q-Box OX1LP Software
“I have tested my new Qubit system with isolated oyster mitochondria, and was extremely pleased with the results. I can’t believe I was suffering for the whole year with my old chamber. I have tried three different systems based on Clarke-type electrodes including this one, and this is by far the most convenient for use with small sample volumes and gives a very clean, repeatable trace with the best signal to noise ratio I ever had with micro-chambers.”
Dr. Inna Sokolova, Assistant Professor, Biology Department, University of North Carolina at Charlotte