S104 DOX Differential O2 Analyzer

Qubit Systems’ S104 Differential O2 Analyzer (DOX) is the world’s only patented differential oxygen analyzer with a resolution of ± 1 ppm O2 against a background of air. With the S104 DOX, researchers can measure real-time rates of respiratory oxygen consumption from tiny insects and photosynthetic oxygen production from a single intact leaf. The S104 Differential O2 Analyzer has the greatest dynamic range of any gas analyzer on the market and on a lower resolution setting it can measure O2 exchange in larger animals, even Human.

When used with a CO2 analyzer (S157) in an open gas exchange system, measurements of Gas Exchange Quotient allow non-invasive studies of respiratory metabolite determination and analyses of reductant flow in anabolic and catabolic reactions.

S104 DOX O2 Analyzer can be employed in human metabolic studies using the room, pod or hood calorimetry approach. For more information visit www.qubitcpx.com.

  • ±1 ppm oxygen resolution against air (that’s 0.0001%!)
  • Widest dynamic range of any oxygen analyzer
  • Easy calibration without special calibration gases
  • Separate analog signals for reference and sample pO2
  • Analog signals for differential pO2, absolute pressure, differential pressure, sample and reference cell temperature, instrument temperature
  • Single Leaf Photosynthetic O2 Exchange
  • Insect Respiratory O2 Consumption
  • Small and Large Animal Respirometry
  • Soil Respiration
  • Leaf Dark Respiration Rate
  • Human Metabolic Studies (Room Calorimetry)
  • Power Supply: 12V 115/220 VAC .
  • Oxygen Sensor life: 3 to 5 years (easily replaceable)
  • Analog Output: 0 to 5 V, Recommend using 16-Bit Analog to Digital converter
  • Absolute Signal range: Reference and Sample  0 to 100% O2
  • Absolute Signal Resolution: 0.001%O2,
  • Absolute Signal Accuracy : +/- 0.002% O2,
  • Absolute Signal Response Time: T90 = 20 seconds, Partial Pressure measurement.
  • Differential Oxygen Signal Range: 1000 to 10000 ppmO2 (user defined),
  • Differential Oxygen Signal Resolution: 1 ppmO2,
  • Differential Oxygen Signal Accuracy: +/- 2.5 ppmO2,
  • Differential Oxygen Signal Response Time: T90 = 20 seconds, Partial Pressure measurement.
  • Absolute Pressure Signal Range: 15 to 115 kPa,
  • Absolute Pressure Signal Resolution: 0.01 kPa,
  • Absolute Pressure Signal Noise < 0.01 kPa,
  • Absolute Pressure Signal Accuracy:  1% of Full Scale.
  • Differential Pressure Signal Range: is +/- 620 Pa,
  • Differential Pressure Signal Resolution: 1 Pa,
  • Differential Pressure Signal Accuracy: 1% of Full Scale.
  • Reference and Sample Air Temperature Signals Range: s 0 to 50 C,
  • Reference and Sample Air Temperature Signals Resolution: 0.01 C,
  • Reference and Sample Air Temperature Signals Accuracy: +/- 0.1 C.
  • Oven Temperature Signal Range: 10 to 50 C,
  • Oven Temperature Signal Resolution:  0.01 C,
  • Oven Temperature Signal Accuracy: +/- 0.1 C.
  • Willms JR, Dowling AN, Dong ZM, Hunt S, Shelp BJ, Layzell DB The simultaneous measurement of low rates of CO2 and O2 exchange in Biological Systems. Anal. Biochem. 254: 272-282 (1997)
  • Willms JR, Salon C, Layzell DB Evidence for light-stimulated fatty acid synthesis in soybean fruit. Plant Physiol. 120: 1117-112 (1999)
  • Cen Y-P, Turpin DH, Layzell DB Whole-plant gas exchange and reductive biosynthesis in white lupin. Plant Physiol. 126: 1555-1565 (2001)
  • Amthor,JS, Koch GW, Willms JR, Layzell DB  Leaf O2 uptake in the dark is independent of coincident CO2 partial pressure. J.Exp.Bot. 52: 2235-2238 (2001)
  • Davey, PA, Hunt S, Hymus GJ, DeLucia EH, Drake BG, Karnosky DF, Long SP Respiratory oxygen uptake is not decreased by an instantaneous elevation of [CO2], but is increased with long term growth in the filed at elevated [CO2]. Plant Physiol. 134: 520-527 (2004)
  • John P. Isanhart, F. M. Anne McNabb and Philip N. Smith. Effects of perchlorate exposure on resting metabolism, peak metabolism, and thyroid function in the prairie vole (Microtus ochrogaster). Environmental Toxicology and Chemistry Vol 24, Issue 3, p678–684 (2005)
  • Efrat Elimelech And Berry Pinshow. Variation in food availability influences prey-capture method in antlion larvae. Ecological Entomology Vol 33, Issue 5, p652–662 (2008).
  • Leakey ADB, Xu F, Gillespie KM, McGrath JM, Ainsworth EA, Ort DR. Genomic basis for stimulated respiration by plants growing under elevated carbon dioxide. PNAS Vol. 106 Number 9 p3597-3602 (2009)

Sample O2 and CO2 data from mealworms

The S104 DOX Differential Oxygen Analyzer in true differential mode has ranges of ±100, ±300 and ±1000 Pa O2. Both the reference and sample oxygen sensors are monitored independently at the same time. An automated Reference – Sample feature provides measurements of any oxygen difference. By choosing the appropriate range setting, oxygen exchange can be measured with any animal.

Most high resolution gas analyzers require calibration with expensive standard gases, or with elaborate mixing systems to produce these gases. Over the years, calibration costs can far exceed the cost of the analyzer itself. The S104 DOX O2 Analyzer has an integrated calibration system that uses ambient air (simple pressure based calibration).  Calibration is simple but extremely accurate, and perfectly linear over the entire dynamic range of the analyzer and the “cal gas” – Air is freely available.

Dr. Bill Shipley, Département de Biologie, Université de Sherbrooke

“When purchasing a new gas exchange system for research purposes, I took a chance with Qubit systems; they claimed to produce a competitive system at almost half the price of those of more established companies. I am happy to say that I am entirely happy with my decision. I now have a reliable and versatile system that is just as accurate as the more expensive competition and, because it is modular, can be expanded as my needs evolve. Most importantly, I received outstanding service during all phases of purchase: conception, ordering, installation and continuing help.”Testimonial